Plasma was separated from the whole blood and lipids were extracted from plasma and analyzed using liquid chromatography. Lipids function as an energy reservoir and serve as major structural components of biological membranes such as plasma membranes. Using liquid chromatography tandem mass spectrometry, we profiled the human plasma lipidome of patients that survived and succumbed to evd during the 2014 outbreak in sierra leone. Pdf human blood is a selfregenerating, lipidrich biologic fluid that is routinely. Methods and results we performed a genomewide association study gwas of 141 lipid species n2,181 individuals, followed by phenomewide scans phewas with 44 clinical endpoints related to cardiometabolic, psychiatric and gastrointestinal. Although such interlipid species correlations have been conceptually implied, their complete characterization is currently lacking. Lipidome is a term coined in the context of omics in modern biology, within the field of lipidomics.
From the departments of medicine and pharmacology o. Genetics of human plasma lipidome and its link to diseases. Plasma was separated from the whole blood and lipids were extracted from plasma and analyzed using liquid. The zipped folder contains two excel files and one. Unlike human plasma quehenberger and dennis 2011 for example, the elf lipidome is dominated by a restricted number of mostly disaturated pc species, including the highly surfaceactive lipid. The ckd plasma lipidome varies with disease severity and. Background nonhuman primates nhp are now being considered as models for investigating human metabolic diseases including diabetes. Expanding lipidome coverage using msmsaided untargeted.
Plasma lipidome reveals critical illness and recovery from. The first cell lipidome to be published was that of a mouse macrophage in 2010. Genetic architecture of human plasma lipidome and its link. Human plasma lipidome is pleiotropically associated with. Aim genetic investigation of human plasma lipidome to get insights into lipidrelated disorders beyond traditional lipid measures. Shared genetic influences reduce the dimensionality of the human lipidome into clusters that are associated with risk factors of cvd. To understand the genetic influence on cvd, it is imperative to understand 1 whether the human plasma lipidome is itself genetically controlled and 2 whether the plasma lipidome and cvd share genetic influences. The lipidome of the yeast saccharomyces cerevisiae has been characterised with an estimated 95% coverage. The emerging development of new analytical mass spectrometry technologies resulted in the possibility of a detailed fractionation of biologically important compounds. Pdf the effect of anticoagulants, temperature, and time.
To better understand human response to a live, attenuated tularemia vaccine and the biological pathways altered postvaccination, healthy adults were vaccinated and plasma was collected pre and postvaccination for longitudinal lipidomics studies. Machine learning of human plasma lipidomes for obesity. Pdf genetic architecture of human plasma lipidome and. The effect of anticoagulants, temperature, and time on the. Here we provide a comprehensive examination of the evd lipidome. Understanding genetic architecture of plasma lipidome could provide better insights into lipid metabolism and its link to cardiovascular diseases.
The effect of anticoagulants, temperature, and time on the human plasma metabolome and lipidome from healthy donors as determined by liquid chromatographymass spectrometry. Obesity is associated with changes in plasma lipids, but while simple lipid quantification is routinely used, plasma lipids are rarely investigated at the level of individual molecules. Comparative plasma lipidome between human and cynomolgus. Genetic investigation of human plasma lipidome to get insights into lipidrelated disorders beyond traditional lipid measures. Analyses of cholesterol and triglycerides in plasma derived from nhps can easily be achieved using methods employed in humans. Understanding genetic architecture of plasma lipidome could provide. A machine learning study based on lipidomes of a total of 1,311 individuals reveals improved associations of plasma lipids with total body fat and fat distribution compared to routine clinical laboratory variables. Abstract human plasma lipidome has been extensively studied in many pathophysiological contexts with the hope of identifying biomarkers for early diagnostics and monitoring the progression and treatment of a broad spectrum of diseases. Human plasma comprises nucleic acids, amino acids mostly in the form of proteins, carbohydrates in the form of monosaccharides and disaccharides, and lipids fig.
A comment on this article appears in the human plasma lipidome. The lipidome refers to the totality of lipids in cells. The employed methodology was applied in a variety of samples, such as humanrodent blood serum and plasma, cells, model organisms such as c. Information pertaining to other lipid species in monkey plasma, however, is lacking and requires comprehensive experimental analysis. Blood was collected in 2015 from sierra leone healthy volunteers and evd patients survivors and fatalities after initial diagnosis, with serial samples collected from survivors over the course of evd and recovery. Download pdf supplementary material xml email share.
The plasma lipidome is dramatically altered by evd. Biochemical analyses reveal that plasma contains over 600 structural forms of. Pdf is available to download from the html version of the article. Download fulltext pdf genetic architecture of human plasma lipidome and its link to cardiovascular disease article pdf available in nature communications 101. Tularemia is a highly infectious and contagious disease caused by bacteria francisella tularensis. It can be studied using mass spectrometry and bioinformatics as well as traditional labbased methods. As applied to lipids, it promoted the development of opportunities for identification of the whole set of lipid molecular species, or the lipidome of a biological object. Lipids are one of the four major molecular components of biological organisms, along with proteins, sugars and nucleic acids. For example, the human plasma lipidome consist of almost 600 distinct molecular species.
Lipidomics unveils the complexity of the lipidome in metabolic. The plasma level of pcs, lpcs, and sms was decreased in hd patients compared with nondialyzed ckd patients all p human plasma lipidome oswald quehenberger, ph. Pdf msbased lipidomics of human blood plasma a community. Understanding genetic architecture of plasma lipidome could provide better insights into lipid metabolism and its link to cardiovascular diseases cvds. Lipidomics of human blood plasma is an emerging biomarker discovery approach that compares lipid profiles under pathological and physiologically normal. Human plasma lipidome has been extensively studied in many pathophysiological contexts with the hope of identifying biomarkers for early diagnostics and.